Correlative Light and Electron Microscopy IV 1st Edition by Thomas Muller Reichert, Paul Verkade ISBN 9780128220580 0128220589

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ISBN 10: 0128220589
ISBN 13: 9780128220580
Author: Thomas Muller Reichert, Paul Verkade

Correlative Light and Electron Microscopy IV, Volume 162, a new volume in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Besides the detailed description of protocols for CLEM technologies including time-resolution, Super resolution LM and Volume EM, new chapters cover Workflow (dis)-advantages/spiderweb, Serial section LM + EM, Platinum clusters as CLEM probes, Correlative Light Electron Microscopy with a transition metal complex as a single probe, SEM-TEM-SIMS, HPF-CLEM, A new workflow for high-throughput screening of mitotic mammalian cells for electron microscopy using classic histological dyes, and more.

• Contains contributions from experts in the field
• Covers topics using nano-SIMS and EDX for CLEM
• Presents recent advances and currently applied correlative approaches
• Gives detailed protocols, allowing for the application of workflows in one’s own laboratory setting
• Covers CLEM approaches in the context of specific applications
• Aims to stimulate the use of new combinations of imaging modalities

Correlative Light and Electron Microscopy IV 1st Edition Table of contents:

Chapter 1: 50 Shades of CLEM: How to choose the right approach for you

Abstract

1: What is CLEM?

2: The microscopist’s dilemma

3: Sample preservation

4: Case studies

5: How to choose your shade of CLEM: Closing remarks

Acknowledgments

Chapter 2: The Histo-CLEM Workflow for tissues of model organisms

Abstract

1: Introduction

2: General design of the Histo-CLEM Workflow

3: Application example

4: Concluding remarks

Acknowledgments

Chapter 3: Fluorescent platinum nanoclusters as correlative light electron microscopy probes

Abstract

1: Introduction

2: CLEM probes

3: Instrumentation, materials, and reagents

4: Methods

5: Generation and validation of the probe

6: Use of PtNCs

7: Discussion and outlook

Acknowledgments

Chapter 4: Refining a correlative light electron microscopy workflow using luminescent metal complexes

Abstract

1: Introduction

2: Transition metal Ir complex 1

3: Instrumentation and materials

4: Methods

5: Results

6: Discussion

Acknowledgments

Chapter 5: Sample preparation for energy dispersive X-ray imaging of biological tissues

Abstract

1: Introduction

2: Routine chemical fixation of tissues

3: Resin

4: Contrasting

5: Grids and sample holder

6: Other considerations

7: Conclusion

8: Materials

Acknowledgments

Author contributions

Chapter 6: HPM live μ for a full CLEM workflow

Abstract

1: Introduction

2: High pressure freezing principles

3: Designing the HPM live μ

4: Integration of the HPM live μ in a CLEM workflow

5: A live imaging, cryo-fluorescence, in-resin fluorescence and EM workflow: Validation at each step

6: Perspectives

Acknowledgments

Author contributions

Chapter 7: High-throughput screening of mitotic mammalian cells for electron microscopy using classic histological dyes

Abstract

1: Introduction

2: Methods

3: Instrumentation and materials

4: Discussion

Acknowledgments

Chapter 8: On-section correlative light and electron microscopy of large cellular volumes using STEM tomography

Abstract

1: Introduction

2: Methods

3: Instrumentation and materials

4: Results

5: Discussion

6: Conclusion

Acknowledgments

Chapter 9: An accelerated procedure for approaching and imaging of optically branded region of interest in tissue

Abstract

1: Introduction

2: Rationale

3: Methods

4: Instrumentation and materials

5: Discussion and conclusion

Acknowledgments

Chapter 10: Cryo-fluorescence microscopy of high-pressure frozen C. elegans enables correlative FIB-SEM imaging of targeted embryonic stages in the intact worm

Abstract

1: Introduction

2: A note on selecting the right cryoprotectant

3: Rationale

4: Methods

5: Instrumentation and materials

6: Discussion

Acknowledgments

Chapter 11: Correlative super-resolution fluorescence and electron cryo-microscopy based on cryo-SOFI

Abstract

1: Introduction

2: Rationale

3: Materials

4: Methods

5: Discussion

Acknowledgments

Chapter 12: Cryo-correlative light and electron microscopy workflow for cryo-focused ion beam milled adherent cells

Abstract

1: Introduction

2: Rationale

3: Materials

4: Methods

5: Results and discussion

Acknowledgments

Chapter 13: Super-resolution correlative light-electron microscopy using a click-chemistry approach for studying intracellular trafficking

Abstract

1: Introduction

2: Methods

3: Instrumentation and materials

4: Discussion

Acknowledgments

Chapter 14: Step-by-step guide to post-acquisition correlation of confocal and FIB/SEM volumes using Amira software

Abstract

1: Introduction

2: Precursor techniques

3: Software and hardware

4: Step-by-step guide

5: Summary and discussion

Acknowledgments

Chapter 15: Visualization and co-registration of correlative microscopy data with the ImageJ plug-in Correlia

Abstract

1: Introduction

2: Models for image co-registration

3: Correlia

4: Two real-life examples

5: Summary and conclusions

Acknowledgments

Chapter 16: Multimodality imaging beyond CLEM: Showcases of combined in-vivo preclinical imaging and ex-vivo microscopy to detect murine mural vascular lesions

Abstract

1: Introduction

2: Rationale

3: Methods and results

4: Discussion

5: Conclusion and outlook

Acknowledgment

Chapter 17: Correlative multimodal imaging: Building a community

Abstract

1: Setting the scene

2: A brief history

3: Building the community

4: Conclusion

Acknowledgments

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Tags: Thomas Muller Reichert, Paul Verkade, Correlative Light, Electron Microscopy IV