Adhesion Protein Protocols 3rd Edition by Amanda S Coutts ISBN 1627035389 9781627035378

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ISBN 10: 1627035389
ISBN 13: 9781627035378
Author: Amanda S Coutts

Adhesion Protein Protocols 3rd Edition Table of contents:

1. Introduction to Cell Adhesion and Adhesion Proteins

• Biology of Cell Adhesion

• Types of Adhesion Molecules (CAMs, Integrins, Cadherins, etc.)

• Role of Adhesion Proteins in Physiology and Disease

• Recent Advances and Applications

2. Sample Preparation and Cell Culture Techniques

• Culturing Adherent and Suspension Cells

• Preparing Tissue Samples for Adhesion Studies

• Coating Substrates with ECM Proteins

• Fixation and Permeabilization Protocols

3. Isolation and Purification of Adhesion Proteins

• Lysis Buffers and Extraction Conditions

• Affinity Chromatography for Adhesion Molecules

• Immunoprecipitation of Adhesion Complexes

• Protein Quantification and Quality Control

4. Protein Interaction Assays

• Co-immunoprecipitation (Co-IP)

• Pull-down Assays

• Cross-linking and Proximity Labeling Techniques

• BiFC and FRET for Live-Cell Analysis

5. Functional Assays for Cell Adhesion

• Static Adhesion Assays

• Flow-Based Adhesion Assays (Microfluidic Systems)

• Cell Spreading and Morphology Assays

• Migration and Invasion Assays

6. Imaging and Visualization Techniques

• Immunofluorescence Staining of Adhesion Proteins

• Confocal and Super-Resolution Microscopy

• Live-Cell Imaging of Adhesion Dynamics

• Image Analysis Software and Quantification

7. Molecular Analysis of Adhesion Proteins

• Western Blotting and Detection

• qPCR for Adhesion Gene Expression

• CRISPR/Cas9 Editing of Adhesion Genes

• siRNA and shRNA Knockdown Protocols

8. High-Throughput and Omics Approaches

• Proteomics of Adhesion Complexes

• Mass Spectrometry-Based Identification

• Bioinformatics Tools for Protein Networks

• RNA-Seq and Single-Cell Analysis of Adhesion Pathways

9. In Vivo Models and 3D Systems

• Organoids and 3D Cultures

• Mouse Models for Adhesion Protein Function

• Zebrafish and Xenopus in Adhesion Research

• Imaging Adhesion in Live Tissues

10. Troubleshooting and Best Practices

• Common Pitfalls in Adhesion Protein Studies

• Ensuring Reproducibility and Valid Controls

• Antibody Validation and Specificity Checks

• Experimental Design Tips

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