Base Editors Methods and Protocols 1st Edition by Sangsu Bae, Beomjong Song ISBN 9781071628782 107162878X

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ISBN 10: 107162878X
ISBN 13: 9781071628782
Author: Sangsu Bae, Beomjong Song

This volume explores base editors (BEs), an invaluable CRISPR-based genome editing tool with a wide variety of versatile applications. Beginning with an overview of BEs, their diverse variants, and computational tools, the book continues with experimental applications of BEs for disease modeling in mammalian cells and generating mutagenic mice, therapeutic base editing strategies, which covers delivery methods of BE-encoded DNA plasmids, mRNAs, or ribonucleoproteins through viruses or non-viral lipid nanoparticles, and lastly, the use of BEs in plants and bacteria. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Base Editors: Methods and Protocols serves as an ideal guide for researchers looking to use base editors to continue their studies in an array of fields.

Base Editors Methods and Protocols 1st Edition Table of contents:

Part I: Overview of Base Editors (BEs), Diverse Variants, and Computational Tools for BEs

1. Introduction and Perspectives of DNA Base Editors
   Beomjong Song and Sangsu Bae

2. Web-Based Computational Tools for Base Editors
   Gue-Ho Hwang and Sangsu Bae

3. Prediction of Base Editing Efficiencies and Outcomes Using DeepABE and DeepCBE
   Jinman Park and Hui Kwon Kim

4. Profiling Genome-Wide Specificity of dCpf1 Cytidine Base Editors Using Digenome-Seq
   Daesik Kim

Part II: Application of BEs for Disease Modeling

5. Base Editing of Human Hematopoietic Stem Cells
   Jing Zeng, Gabriele Casirati, My Anh Nguyen, Pietro Genovese, and Daniel E. Bauer

6. A/C Simultaneous Conversion Using the Dual Base Editor in Human Cells
   Xiaohui Zhang, Yuting Guan, and Dali Li

7. Functional Analysis of Variants in BRCA1 Using CRISPR Base Editors
   Ji-Eun See and Yongsub Kim

8. Use of the Representative Base Editing Tool Target-AID to Introduce Pathogenic Mutations into Mice
   Hiroki Sasaguri

9. Targeted Mutagenesis in Mice Using a Base Editor
   Tae Yeong Jeong, Soo-Yeon Lim, Je Kyung Seong, and Kyoungmi Kim

Part III: Therapeutic Base Editing Strategies

10. Heterologous Expression and Purification of a CRISPR-Cas9-Based Adenine Base Editor
    Seu-Na Lee, Hong-Su Jang, and Jae-Sung Woo

11. Delivering Base Editors In Vivo by Adeno-Associated Virus Vectors
    Colin K.W. Lim, Angelo J. Miskalis, Pablo Perez-Pinera, and Thomas Gaj

12. The Delivery of ABE mRNA to the Adult Murine Liver by Lipid Nanoparticles (LNPs)
    Dong Yang and Chun-Qing Song

13. Ex Vivo Base Editing Therapy with Chemically-Derived Hepatic Progenitors
    Yohan Kim, Jaemin Jeong, and Dongho Choi

14. Application of Base Editor-Mediated Genome Editing in Mouse Retina
    Jun Wu, Chang Sik Cho, Dong Hyun Jo, and Jeong Hun Kim

Part IV: Use of BEs in Plants and Bacteria

15. High-Throughput Base Editing-Mediated Artificial Evolution Streamlines Trait Gene Identification in Rice
    Fang Yan, Man Yu, Meixia Wang, and Huanbin Zhou

16. Heritable Virus-Induced Genome Editing (VIGE) in Nicotiana attenuata
    Hyeonjin Kim, Youngbin Oh, Eunae Park, Moonyoung Kang, Yuri Choi, and Sang-Gyu Kim

17. Cytosine Base Editing in Bacteria
    [Author not specified in the provided information]

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