Cell Penetrating Peptides Methods and Protocols 3rd Edition by Ulo Langel ISBN 1071617516 9781071617519

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Product details:

ISBN 10: 1071617516

ISBN 13: 978-1071617519

Author:  Ülo Langel 

Cell Penetrating Peptides: Methods and Protocols 3rd Edition: This third edition summarizes the most important areas of Cell-Penetrating Peptides (CPP) research. Chapters are divided into three parts detailing the historical background of CPP, the classifications of the available CPPs, approaches for prediction of novel CPPs, methods for studies of “naked” CPPs, and a brief summary of functionality issues of CPPs, both in vitro and in vivo. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

Authoritative and cutting-edge, Cell Penetrating Peptides: Methods and Protocols, Third Edition aims to be a useful practical guide to researches to help further their study in this field.

 

Cell Penetrating Peptides: Methods and Protocols 3rd Edition Table of contents:

PART I INTRODUCTION

1. Classes of Cell-Penetrating Peptides
2. Penetratin Story: An Overview
3. Prediction of Cell-Penetrating Peptides
4. Computer-Aided Virtual Screening and Designing of Cell-Penetrating Peptides

PART II METHODS TO TEST MECHANISMS OF CELL-PENETRATING PEPTIDES
5. Investigating Membrane Interactions and Structures of CPPs
6. Determining the Effects of Membrane-Interacting Peptides on Membrane Integrity
7. Study of CPP Mechanisms by Mass Spectrometry
8. Methods to Study the Role of the Glycocalyx in the Uptake of Cell-Penetrating Peptides
9. Toxicity, Immunogenicity, Uptake, and Kinetics Methods for CPPs
10. Unraveling the Mechanisms of Peptide-Mediated Delivery of Nucleic Acids Using Electron Microscopy
11. SCARA Involvement in the Uptake of Nanoparticles Formed by Cell-Penetrating Peptides

PART III APPLICATIONS OF CPPS IN VITRO AND IN VIVO
12. Protein Mimicry and the Design of Bioactive Cell-Penetrating Peptides
13. Pepducins and Other Lipidated Peptides as Mechanistic Probes and Therapeutics
14. Identification and Characterization of Homing Peptides Using In Vivo Peptide Phage Display
15. The Antimicrobial and Antiviral Applications of Cell-Penetrating Peptides
16. Visualizing Actin Architectures in Cells Incubated with Cell-Penetrating Peptides
17. Cell-Penetrating Peptides as Carriers for Transepithelial Drug Delivery In Vitro
18. A Pathway Toward Tumor Cell-Selective CPPs
19. PepFects and NickFects for the Intracellular Delivery of Nucleic Acids
20. In Vitro Assays to Assess Exon Skipping in Duchenne Muscular Dystrophy
21. Applications of ApoB LDLR-Binding Domain Approach for the Development of CNS-Penetrating Peptides for Alzheimer’s Disease
22. CPP-Based Delivery System for In Vivo Gene Delivery
23. Application of CPPs for Brain Delivery
24. Intracellular Delivery of Nanoparticles with Cell Penetrating Peptides
25. Multifunctional Oligoaminoamides for the Receptor-Specific Delivery of Therapeutic RNA
26. Cell Penetrating Peptides for Chemical Biological Studies
27. Experiences with CPP-Based Self-Assembling Peptide Systems for Topical Delivery of Botulinum Toxin
28. Applications of CPPs in Genome Modulation of Plants
29. DNA Transfer into Animal Cells Using Stearylated CPP-Based Transfection Reagent
30. Live Cell Genomics: Cell-Specific Transcriptome Capture in Live Tissues and Cells
31. Live Cell Genomics: RNA Exon-Specific RNA-Binding Protein Isolation

 

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