Cell Viability Assays Methods and Protocols Methods in Molecular Biology 2644 1st editon by Oliver Friedrich, Daniel Gilbert 1071630547 978-1071630549

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ISBN 10: 1071630547
ISBN 13: 978-1071630549
Author:  Oliver Friedrich, Daniel F. Gilbert

This updated edition explores assessing cell viability as a measure for cell fitness under conditions of physiological and patho-physiological stress as well as challenging conditions to cellular and tissue homeostasis, and accounts for the ongoing 2D-to-3D development with topics and assays that target cell viability, mobility, and functionality of tissues and organs, natural or bioartificial, in 3D. The book’s contents span a wide range of viability and functionality assays, from impedance spectroscopy to chemiluminescence, fluorescence and label-free optical detection methodologies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and up-to-date, Cell Viability Assays: Methods and Protocols, Second Edition serves as a valuable resource to the growing community in bioinspired life sciences, biomedical sciences, and biotechnology by providing more standardized protocols to probe the “wellbeing” of cells in various environments.

Cell Viability Assays Methods and Protocols Methods in Molecular Biology 2644 1st Table of contents:

1. Front Matter
2. Assaying Mitochondrial Respiration as an Indicator of Cellular Metabolism and Fitness
3. The MTT Assay: A Method for Error Minimization and Interpretation in Measuring Cytotoxicity and Estimating Cell Viability
4. Assaying Proliferation Characteristics of Cells Cultured Under Static Versus Periodic Conditions
5. Network Reconstruction as a Novel High-Level Marker of Functional Neuronal Viability
6. Assaying Mitochondrial Function by Multiparametric Flow Cytometry
7. High-Efficiency Single-Cell Electrical Impedance Spectroscopy
8. Cell Viability Multiplexing: Quantification of Cellular Viability by Barcode Flow Cytometry and Computational Analysis
9. Average Rheological Quantities of Cells in Monolayers
10. Assaying Spontaneous Network Activity and Cellular Viability Using Multi-Well Microelectrode Arrays
11. Quantitative Live-Cell Ca2+ Imaging During Isotropic Cell Stretch
12. Assessment of Cell Viability in Electrically Excitable Muscle Cells Through Intact Twitch Stimulation
13. Evaluating Cellular Viability by iTRAQ Proteomic Profiling
14. Quantifying pH in Malaria Using pHluorin and Flow Cytometry
15. Cell Membrane State, Permeability, and Elasticity Assessment for Single Cells and Cell Ensembles
16. Neutral Red Uptake Assay to Assess Cytotoxicity In Vitro
17. Digital Holographic Microscopy to Assess Cell Behavior
18. Second Harmonic Generation Morphometry of Muscle Cytoarchitecture in Living Cells
19. Optimization of Cell Viability Assays for Drug Sensitivity Screens

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Oliver Friedrich,Daniel Gilbert,Cell Viability Assays Methods