Directed Evolution Methods and Protocols Methods in Molecular Biology 2461 by Andrew 97810716215161 071621513

Directed Evolution Methods and Protocols Methods in Molecular Biology 2461 Andrew Currin (Editor) – Ebook Instant Download/Delivery ISBN(s): 9781071621516,1071621513

Product details:

• ISBN 10:071621513
• ISBN 13: 97810716215161
• Author:  Andrew 

This volume explores the latest techniques used by researchers to study directed evolution (DE) at each stage of the Design-Build-Test-Learn cycle. Chapters in this book cover topics such as designing overlap extension PCR primers for protein mutagenesis; antha-guided automation of Darwin assembly for the construction of bespoke gene libraries; rapid cloning of random mutagenesis libraries using PTO-Quickstep; and DE of glycosyltransferases by a single-cell screening method. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Directed Evolution: Methods and Protocols is a valuable resource for scientists and researchers who are interested in learning more about this field and incorporating these studies into new experimental workflows.

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