Genome Editing Current Technology Advances and Applications for Crop Improvement 1st Edition by Shabir Hussain Wani 9783031080722 3031080726

Genome Editing Current Technology Advances and Applications for Crop Improvement 1st Edition Shabir Hussain Wani – Ebook Instant Download/Delivery ISBN(s): 9783031080722, 3031080726

Product details:

• ISBN 10:3031080726
• ISBN 13:9783031080722
• Author: Shabir Hussain Wani 

Genome Editing
Current Technology Advances and Applications for Crop Improvement
Over the last few decades, various techniques have been developed to alter the properties of plants and animals. While the targeted transfer of recombinant DNA into crop plants remains a valuable tool to achieve a desirable breeding outcome, integration of transgenes into the host genome has been random, which in part, leads to reduced acceptance of GMOs by the general population in some parts of the world. Likewise, methods of induced mutagenesis, such as TILLING, have the disadvantage that many mutations are induced per plant, which has to be removed again by expensive backcrossing. Advances in genome sequencing have provided more and more information on differences between susceptible and resistant varieties, which can now be directly targeted and modified using CRISPR/Cas9 technology. By selecting specific gRNAs occurrence of off-target modifications are comparatively low. ZFNs and TALENs- based approaches required re-engineering a new set of assembled polypeptides for every new target site for each experiment. The difficulty in cloning and protein engineering prevented these tools from being broadly adopted by the scientific community. Compared to these technologies, designing the CRISPR toolbox is much simpler and more flexible. CRISPR/Cas9 is versatile, less expensive and highly efficient. It has become the most widely used technology for genome editing in many organisms. Since its inception as a powerful genome-editing tool in late 2012, this breakthrough technology has completely changed how science is performed. The first few chapters in this book introduce the basic concept, design and implementation of CRISPR/Cas9 for different plant systems. They are followed by in-depth discussions on the legal and bio-safety issues accompanying commercialization and patenting of this emerging technology. Lastly, this book covers emerging areas of new tools and potential applications. We believe readers, novice and expert alike, will benefit from this all-in-one resource on genome editing for crop improvement. Chapter 17 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com

Table contents:

Part I. Current Status and Challenges of Plant Genome Editing Using CRISPR/Cas Technology
Genome Engineering as a Tool for Enhancing Crop Traits: Lessons from CRISPR/Cas9
Vegetable Crop Improvement Through CRISPR Technology for Food Security
CRISPR/Cas9-Mediated Targeted Mutagenesis in Medicinal Plants
Genome Editing: A Review of the Challenges and Approaches
Part II. Developments in the Field of CRISPR/Cas Technology
Recent Advances and Application of CRISPR Base Editors for Improvement of Various Traits in Crops
New Cas Endonuclease Variants Broadening the Scope of the CRISPR Toolbox
Multiplexed Genome Editing in Plants Using CRISPR/Cas-Based Endonuclease Systems
Transgene-Free Genome Editing in Plants
Genome Editing by Ribonucleoprotein Based Delivery of the Cas9 System in Plants
Virus-Mediated Delivery of CRISPR/CAS9 System in Plants
Characterization of Gene Edited Crops via Metabolomics
Part III. Applications of CRISPR/Cas Technology for Biotic and Abiotic Stress Tolerance
Genome Editing in Plants for Resistance Against Bacterial Pathogens
Improvement of Resistance in Plants Against Insect-Pests Using Genome Editing Tools
Applications of Gene Drive for Weeds and Pest Management Using CRISPR/Cas9 System in Plants
Recent Trends in Targeting Genome Editing of Tomato for Abiotic and Biotic Stress Tolerance
Part IV. Legal Framework for CRISPR/Cas Technology
Biosafety Issue Related to Genome Editing in Plants Using CRISPR-Cas9
Regulatory Constraints and Differences of Genome-Edited Crops Around the Globe
Correction to: Multiplexed Genome Editing in Plants Using CRISPR/Cas-Based Endonuclease Syste

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