Microtubules Methods and Protocols 1st edition by Hiroshi Inaba 1071619853 9781071619858

1. Tubulin Purification and Modification

• Purification of Tubulin from Porcine Brain and its Fluorescence Dye Modification
• Facile Method of Tubulin Purification from Goat Brain for Reconstitution of Microtubule-Associated Intracellular Function
• Functionalization of Tubulin: Approaches to Modify Tubulin with Biotin and DNA

2. Tubulin Properties and Dynamics

• Electro-Modulation of Tubulin Properties and Function
• In Vitro Reconstitution of Microtubule Dynamics and Severing Imaged by Label-Free Interference Reflection Microscopy
• Visualization and Quantification of Microtubule Self-repair

3. Microscopy and Imaging Techniques

• Characterizing the Number of Kinesin Motors Bound to Microtubules in the Gliding Motility Assay using FLIC Microscopy
• Linear-Zero Mode Waveguides for Single-Molecule Fluorescence Observation of Nucleotides in Kinesin-Microtubule Motility Assay
• Real-Time Imaging of Single γTuRC-Mediated Microtubule Nucleation Events In Vitro by TIRF Microscopy
• Microtubule Preparation for Investigation with High-Speed Atomic Force Microscopy
• Cryo-EM Visualization of Neuronal Particles Inside Microtubules

4. Microtubule Engineering and Functionalization

• Design of Mechanical and Electrical Properties for Multi-Directional Control of Microtubules
• Microtubules and Quantum Dots Integration Leads to Conjugates with Applications in Biosensors and Bionanodevices
• Encapsulation of Nanomaterials Inside Microtubules by Using a Tau-derived Peptide
• Crystallization Systems for the High-Resolution Structural Analysis of Tubulin-Ligand Complexes

5. Applications in Active Matter and Robotics

• Assembling Microtubule-Based Active Matter
• Spontaneous Alignment of Microtubules via Tubulin Polymerization in a Narrow Space under a Temperature Gradient
• Dynamic Pattern Formation of Active Matters Triggered by Mechanical Stimuli
• Spontaneously Beating Biomimetic Structures
• Construction of Molecular Robots from Microtubules for Programmable Swarming
• Fabrication of Artificial Muscle from Microtubules, Kinesins, and DNA Origami Nanostructures

6. Investigating Tubulin Interactions and Drug Effects

• Investigating Tubulin-Drug Interaction Using Fluorescence Spectroscopy
• Monitoring the Disruptive Effects of Tubulin Binding Agents on Cellular Microtubules
• Phoontrolling Microtubule Dynamics with Photoswitchable Chemical Reagents

7. Microtubule Transport and Cellular Integration

• Cargo Transport by Microtubule-Associated Motor Protein Along Mechanically Deformed Microtubules
• Mechanical Deformation of Microtubules on a Two-Dimensional Elastic Medium
• Reconstituting the Interaction Between Purified Nuclei and Microtubule Network

8. Advanced Fluorescence and Probing

• Pacific Blue-Taxoids as Fluorescent Molecular Probes of Microtubules

9. Cellular Structure and Patterning

• Controlling Cell Shape and Microtubule Organization by Extracellular Matrix Micropatterning