Mycobacteria Protocols 4th edition by Tanya Parish, Anuradha Kumar ISBN 1071614622  978-1071614624

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ISBN 10: 1071614622
ISBN 13: 978-1071614624
Author: Tanya Parish, Anuradha Kumar

This fully updated edition explores the latest techniques to study the challenging, and at times dangerous, genus of bacteria known as mycobacteria with basic methods that are still required for mycobacteriology along with the newer or improved methods that have been developed. The volume features chapters on the basics of DNA isolation, protein isolation, and lipid isolation, as well as more sophisticated techniques for isolation of ribosomes, and continues with sections involving analyzing subcellular fractions, culture methods, sequencing technology, in vitro models, molecular methods, as well as drug discovery applications. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and up-to-date, Mycobacteria Protocols, Fourth Edition serves as an ideal guide for those starting out with their mycobacteria research and also for those who have worked with it for decades.

Mycobacteria Protocols 4th Table of contents:

Preface

• Acknowledgments
• Introduction to Mycobacteria and the Scope of the Book
• Overview of the 4th Edition

Chapter 1: Introduction to Mycobacteria

• Basic Characteristics of Mycobacteria
• Classification and Species of Mycobacteria
• The Role of Mycobacteria in Human Disease
• Mycobacterial Infections: Tuberculosis and Non-Tuberculous Mycobacteria

Chapter 2: General Laboratory Practices for Mycobacteria

• Safety Considerations and Biosafety in Mycobacterial Work
• General Laboratory Setup for Mycobacteria Cultures
• Standard Laboratory Practices in Mycobacteriology
• Preparation of Culture Media for Mycobacteria

Chapter 3: Isolation and Cultivation of Mycobacteria

• Methods for Culturing Mycobacteria: Solid and Liquid Media
• Use of the BACTEC System for Mycobacterial Growth Detection
• Selective Cultures for Mycobacteria Isolation
• Handling and Maintenance of Mycobacterial Cultures
• Growth Conditions and Incubation Techniques

Chapter 4: Molecular Identification of Mycobacteria

• PCR Techniques for Mycobacterial Species Identification
• Genotyping and Sequencing Methods (16S rRNA, IS6110)
• Mycobacterial DNA Extraction Protocols
• Hybridization and Probe-Based Detection Methods
• The Role of Mass Spectrometry in Mycobacterial Identification

Chapter 5: Phenotypic Identification and Characterization

• Acid-Fast Staining: Ziehl-Neelsen and Kinyoun Methods
• Biochemical Identification of Mycobacteria
• Use of the NAP Test for Mycobacterium tuberculosis
• Other Differential Tests for Mycobacterial Species
• Identification of Non-Tuberculous Mycobacteria (NTM)

Chapter 6: Antimicrobial Susceptibility Testing

• Methods for Antimicrobial Susceptibility Testing in Mycobacteria
• The Proportion Method and Agar Proportions for Mycobacterial Resistance Testing
• Liquid Culture-Based Methods for Drug Sensitivity Testing
• Molecular Methods for Resistance Detection (Gene Mutation Analysis)
• Testing for Drug Resistance in Mycobacterium tuberculosis

Chapter 7: Mycobacteria in Clinical Specimens

• Processing and Decontamination of Clinical Specimens (Sputum, Blood, etc.)
• Methods for Detecting Mycobacteria in Body Fluids
• Quantification of Mycobacteria in Clinical Specimens
• Molecular Detection in Clinical Samples
• Smear Microscopy and Culture Results Interpretation

Chapter 8: Detection and Diagnosis of Mycobacterial Infections

• Laboratory Diagnosis of Tuberculosis: Smear Microscopy, Culture, and PCR
• Diagnosing Non-Tuberculous Mycobacterial Infections
• Liquid Culture Systems in Mycobacteriology
• Role of Immunodiagnostic Tests in Mycobacterial Infections
• Diagnostic Protocols for Drug-Resistant Tuberculosis

Chapter 9: Mycobacterial Pathogenesis and Host Interaction

• Mechanisms of Mycobacterial Pathogenesis
• The Role of Host Immune Responses in Mycobacterial Infections
• Mycobacterial Virulence Factors
• The Tuberculosis Granuloma and Its Role in Disease Progression
• Animal Models for Studying Mycobacterial Pathogenesis

Chapter 10: Advances in Mycobacterial Research and Therapeutics

• New Frontiers in Mycobacterial Drug Development
• The Role of Vaccine Development in Mycobacterial Control
• Recent Advances in Immunotherapy for Mycobacterial Diseases
• CRISPR/Cas9 and Gene Editing in Mycobacterial Research
• Future Trends in Mycobacteriology

Chapter 11: Environmental Mycobacteria and Public Health

• Mycobacteria in the Environment: Water, Soil, and Air
• Control Measures for Environmental Mycobacteria
• Non-Tuberculous Mycobacteria as Emerging Pathogens
• Public Health Surveillance and Reporting of Mycobacterial Infections
• Global Health Challenges: TB and NTM Infections

Chapter 12: Mycobacteria Protocols for Specialized Applications

• Protocols for Mycobacteria in Veterinary Medicine
• Mycobacteria Research in Tuberculosis Control Programs
• Mycobacteria as Bioterrorism Agents: Detection and Response
• Specialized Protocols for High-Containment Laboratories
• Emerging Techniques for Mycobacterial Genetic Engineering

Appendices

• Appendix A: Laboratory Materials and Reagents for Mycobacteria Work
• Appendix B: Troubleshooting Common Mycobacterial Laboratory Problems
• Appendix C: Mycobacteria Databases and Online Resources
• Appendix D: Recommended Reading and Further Resources

Index

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