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Mycobacterium ulcerans Methods and Protocols 1st Edition by Gerd Pluschke, Katharina Röltgen ISBN 1071617818 9781071617816

Name: Mycobacterium ulcerans Methods and Protocols 1st Edition by Gerd Pluschke, Katharina Röltgen ISBN 1071617818 9781071617816
SKU: EB-35986054
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Mycobacterium ulcerans Methods and Protocols 1st Edition Gerd Pluschke Katharina Röltgen Digital Instant Download

Author(s): Gerd Pluschke Katharina Röltgen

Edition: 1

File Details: EPUB, 15.43 MB

Year: 2021

Language: english

SKU: EB-35986054 Category: Medicine Tag: Gerd Pluschke Katharina Röltgen

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Mycobacterium ulcerans Methods and Protocols 1st Edition by Gerd Pluschke, Katharina Röltgen – Ebook PDF Instant Download/Delivery: 1071617818 ,9781071617816
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ISBN 10: 1071617818
ISBN 13: 9781071617816
Author: Gerd Pluschke, Katharina Röltgen

This volume explores the latest techniques used to study Mycobacterium ulcerans, and more specifically M. ulcerans disease (Buruli ulcer). The chapters in this book are organized into three parts and cover methods for the detection of M. ulcerans and the analysis of host-pathogen interaction; the quantification and characterization of mycolactone, the macrolide toxin of M. ulcerans; and drug development against M. ulcerans. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Mycobacterium ulcerans: Methods and Protocols is a valuable resource that helps scientists advance their research on Buruli ulcer, which is still an under-researched field in infection biology.

Mycobacterium ulcerans Methods and Protocols 1st Edition Table of contents:

Overview: Mycobacterium ulcerans Disease (Buruli Ulcer)
Katharina Röltgen, Gerd Pluschke

Immunohistochemistry: A Method to Analyze M. ulcerans Infected Skin Tissue
Marie-Thérèse Ruf

Primary Isolation of Mycobacterium ulcerans
Dorothy Yeboah-Manu, Adwoa Asante-Poku, Sammy Yaw Aboagye

Investigation of Mycobacterium ulcerans Glycan Interactions Using Glycan Array and Surface Plasmon Resonance
Christopher J. Day, Jessica Poole, Gerd Pluschke, Michael P. Jennings

Purification and Characterization of Extracellular Vesicles from Mycobacterium ulcerans Culture
Mélanie Foulon, Estelle Marion, Laurent Marsollier

DNA Extraction from Clinical Specimens for the Direct Detection of Mycobacterium ulcerans by Real-Time PCR
Caroline J. Lavender, Janet A. M. Fyfe

Extraction of DNA from Environmental Samples for the Real-Time PCR Detection of Mycobacterium ulcerans
Janet A. M. Fyfe, Caroline J. Lavender

Detection of Mycobacterium ulcerans DNA Using Real-Time PCR
Janet A. M. Fyfe, Caroline J. Lavender

Determining Viability of M. ulcerans by 16S rRNA RT Reverse Transcriptase Real-Time PCR
Marcus Beissner, Richard Odame Phillips, Gisela Bretzel

Methods and Approaches for Buruli Ulcer Surveillance in Africa: Lessons Learnt and Future Directions
Joseph W. S. Timothy, Rachel L. Pullan, Rie R. Yotsu

Purification and Quantification of Myolactone

Overview: Mycolactone, the Macrolide Toxin of Mycobacterium ulcerans
Katharina Röltgen, Gerd Pluschke

Conditions for Handling and Optimal Storage of Mycolactone
Jessica Z. Kubicek-Sutherland, Basil I. Swanson, Harshini Mukundan

Mycolactone Purification from M. ulcerans Cultures and HPLC-Based Approaches for Mycolactone Quantification in Biological Samples
Aline Rifflet, Caroline Demangel, Laure Guenin-Macé

Detection of Mycolactone by Thin Layer Chromatography
Richard Kwamla Amewu, Thomas Spangenberg

Competitive ELISA for the Detection and Quantification of Mycobacterium ulcerans Mycolactone
Louisa Warryn

Biochemical and Biological Assays of Mycolactone-Mediated Inhibition of Sec61
Sarah O’Keefe, Stephen High, Caroline Demangel

Drug Development Against Mycobacterium ulcerans

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Tags: Gerd Pluschke, Katharina Röltgen, Mycobacterium ulcerans, Protocols