NEURONAL CELL CULTURE methods and protocols 2nd Edition by Shohreh Amini, Martyn White 1071614363 9781071614365

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ISBN 10: 1071614363
ISBN 13: 9781071614365
Author: Shohreh Amini, Martyn K White

This second edition volume details the latest aspects of neural cells covering the practical and theoretical considerations of each techniques involved. Chapters guide readers through a general overview of the neuronal culturing principles, cell line models for neural cells, the isolation and propagation of primary cultures, stem cells, transfection and transduction of neural cultures, and other more advanced techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Practical and easy to use, Neuronal Cell Culture: Methods and Protocols, Second Edition aims to be of interest to scientists at all levels studying cell culture models for neuroscientific studies.

NEURONAL CELL CULTURE methods and protocols 2nd Table of contents:

General Overview of Neuronal Cell Culture.

– Considerations for the Use of SH-SY5Y Neuroblastoma Cells in Neurobiology.

– Cultured Cell Line Models of Neuronal Differentiation: NT2, PC12.

– Murine Teraarcinoma-Derived Neuronal Cultures.

– Isolation and Propagation of Primary Human and Rodent Embryonic Neural Progenitor Cells and Cortical Neurons.

– Mouse Enteric Neuronal Cell Culture.

– Preparation of Neural Stem Cells and Progenitors: Neuronal Production and Grafting Applications.

– Derivation of Neuronal Cells from Fetal Normal Human Astrocyte (NHA).

– Slice Culture Modeling of Central Nervous System (CNS) Viral Infection.

– Neurospheres and Glial Cell Cultures: Immunocyhemistry for Cell Phenotyping.

– Transfection of Neuronal Cultures.- Lentiviral Transduction of Neuronal Cells.

– Compartmentalized Neuronal Cultures.

– Quantitative Assessment of Neurite Outgrowth Over Growth Promoting or Inhibitory Substrates.

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