Expression Purification and Structural Biology of Membrane Proteins Methods in Molecular Biology 2127 1st edition by Camilo, Timm 9781071603727 1071603728

Expression Purification and Structural Biology of Membrane Proteins Methods in Molecular Biology 2127 Camilo Perez (Editor) – Ebook Instant Download/Delivery ISBN(s): 9781071603727,1071603728

Product details:

• ISBN 10: 1071603728
• ISBN 13: 9781071603727
• Author: Camilo, Timm

This book collects up-to-date advanced protocols and advice from leading experts in the area of membrane protein biology that can be applied to structural and functional studies of any membrane protein system. The contents explore methods for cloning and expression of membrane proteins and membrane protein complexes in prokaryotic and eukaryotic systems, approaches for protein purification, nanobody applications, as well as biophysical characterization and much more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Table Contents:

1. Cloning and Multi-Subunit Expression of Mitochondrial Membrane Protein Complexes in Saccharomyces cerevisiae
2. Membrane Protein Production in Escherichia coli
3. Membrane Protein Production in Lactococcus lactis for Structural Studies
4. Expression and Purification of Membrane Proteins in Saccharomyces cerevisiae
5. Membrane Protein Expression in Insect Cells Using the Baculovirus Expression Vector System
6. Membrane Extracts from Plant Tissues
7. Membrane Protein Solubilization and Quality Control: An Example of a Primary Active Transporter
8. GPCR Solubilization and Quality Control
9. Affinity Purification of Membrane Proteins
10. Purification of Membrane Proteins by Affinity Chromatography with On-Column Protease Cleavage
11. Biotinylation of Membrane Proteins for Binder Selections
12. Production and Application of Nanobodies for Membrane Protein Structural Biology
13. Identifying Conformation-Selective Heavy-Chain-Only Antibodies Against Membrane Proteins by a Thermal-Shift Scintillation Proximity Assay
14. Reconstitution of Membrane Proteins into Platforms Suitable for Biophysical and Structural Analyses
15. Reconstitution of Proteoliposomes for Phospholipid Scrambling and Nonselective Channel Assays
16. Membrane Protein Cryo-EM: Cryo-Grid Optimization and Data Collection with Protein in Detergent
17. Single-Particle Cryo-EM of Membrane Proteins in Lipid Nanodiscs
18. Fast Small-Scale Membrane Protein Purification and Grid Preparation for Single-Particle Electron Microscopy
19. Stabilization and Crystallization of a Membrane Protein Involved in Lipid Transport
20. In Meso In Situ Serial X-Ray Crystallography (IMISX): A Protocol for Membrane Protein Structure Determination at the Swiss Light Source
21. Membrane Protein Preparation for Serial Crystallography Using High-Viscosity Injectors: Rhodopsin as an Example
22. Hydrogen/Deuterium Exchange Mass Spectrometry for the Structural Analysis of Detergent-Solubilized Membrane Proteins
23. Mechanical Unfolding and Refolding of Single Membrane Proteins by Atomic Force Microscopy
24. Sample Preparation and Technical Setup for NMR Spectroscopy with Integral Membrane Proteins
25. Exploring Lipid and Membrane Protein Dynamics Using Lipid-Bilayer Nanodiscs and Solution-State NMR Spectroscopy

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