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Ferroptosis Methods and Protocols Methods in Molecular Biology 2712 Guido Kroemer (Editor)

Name: Ferroptosis Methods and Protocols Methods in Molecular Biology 2712 Guido Kroemer (Editor)
SKU: EB-51579970
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Ferroptosis Methods and Protocols Methods in Molecular Biology 2712 Guido Kroemer (Editor) Digital Instant Download

Author(s): Guido Kroemer (editor), Daolin Tang (editor)

ISBN(s): 9781071634325, 1071634321

Edition: 1st ed. 2023

File Details: PDF, 6.15 MB

Year: 2023

Language: English

SKU: EB-51579970 Category: Biology and other natural sciences - Molecular Tags: 1071634321, 9781071634325, Daolin Tang (editor), Ferroptosis, Guido Kroemer (editor), Molecular Biology

Description

Ferroptosis Methods and Protocols Methods in Molecular Biology 2712 Guido Kroemer (Editor) – Ebook Instant Download/Delivery ISBN(s): 9781071634325,1071634321

Product details:

• ISBN 10:1071634321
• ISBN 13:9781071634325
• Author(s): Guido Kroemer (editor), Daolin Tang (editor)

This volume provides a comprehensive collection of experimental protocols for investigating ferroptosis in different systems, including cultured cells, animal models, and human tissues. The techniques covered in this book look at various aspects of ferroptosis ranging from the detection of lipid peroxidation to the measurement of glutathione peroxidase activity and the evaluation of mitochondrial morphology. Chapters also discuss basic molecular biology methods such as quantitative PCR and immunoblotting, and advanced imaging techniques such as transmission electron microscopy and confocal fluorescence microscopy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Table contents:

Fluorogenic Probes for Intracellular Iron Detection

Stratifying Ferroptosis Sensitivity in Cells and Tissues with PALP

ChIP and ChIRP Assays in Ferroptosis

PAR-CLIP Assay in Ferroptosis

Organoids Models of Pancreatic Duct Adenocarcinoma

Probing Lipid Peroxidation in Ferroptosis: Emphasizing the Utilization of C11-BODIPY-Based Protocols

Membrane Integrity Assay in Ferroptosis

LC-MS-Based Redox Phosphoipidomics Analysis in Ferroptosis

Monitoring Lysosome Function in Ferroptosis

Monitoring Mitochondria Function in Ferroptosis

Generation of Organoids and Analysis of Ferroptosis in Organoids

Analysis of Protein Degradation in Ferroptosis

Lipidomics Analysis in Ferroptosis

In-Cell Western Assay in Ferroptosis

Flow Cytometric Analysis of Regulated Cell Death

Thermal Shift Assay in Ferroptosis

Detection of Ferroptosis in Patient-Derived Tumor Models

Assessment of Ferroptosis in Hematopoietic Stem and Progenitor Cells

Detection of Ferroptosis by Immunohistochemistry and Immunofluorescence

Analysis of MicroRNAs in Ferroptosis

Detection of Ferroptosis in Models of Brain Diseases

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