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G Protein Coupled Receptor Signaling in Plants 1st edition by Mark Running 1627035311 978-1627035316

Name: G Protein Coupled Receptor Signaling in Plants 1st edition by Mark Running 1627035311 978-1627035316
SKU: EB-47776830
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G Protein Coupled Receptor Signaling in Plants Mark P Running Digital Instant Download

Author(s): Mark P Running

Edition: 2013th

File Details: PDF, 2.77 MB

Year: 2013

Language: english

SKU: EB-47776830 Category: Mathematics Tag: Mark P Running

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G Protein Coupled Receptor Signaling in Plants 1st edition by Mark Running – Ebook PDF Instant Download/Delivery: 1627035311, 978-1627035316
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ISBN 10: 1627035311
ISBN 13: 978-1627035316
Author: Mark P. Running

Remarkably, while G protein-coupled receptors (GPCRs) are highly prevalent in animals and yeast, very few candidate GPCRs have been identified in plants. In G Protein-Coupled Receptor Signaling in Plants: Methods and Protocols, experts in the field describe techniques used in the study of small GTPases and related proteins. Beginning with a chapter on bioinformatics approaches for GPCR discovery, this detailed volume continues with chapters on heterotrimeric G protein subunits, Rab-GTPases, as well as lipid modifications, including myristoylation, acylation, and prenylation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Practical and dependable, G Protein-Coupled Receptor Signaling in Plants: Methods and Protocols aims to aid further studies into the roles of small GTPases which will help elucidate numerous key processes in plants.

G Protein Coupled Receptor Signaling in Plants 1st Table of contents:

Topology Assessment, G Protein-Coupled Receptor (GPCR) Prediction, and In Vivo Interaction Assays to Identify Plant Candidate GPCRs (Pages 1-12)
Measurement of GTP-Binding and GTPase Activity of Heterotrimeric Gα Proteins (Pages 13-20)
Biochemical Analysis of the Interaction Between Phospholipase Dα1 and GTP-Binding Protein α-Subunit from Arabidopsis thaliana (Pages 21-35)
Analysis of Cell Division and Cell Elongation in the Hypocotyls of Arabidopsis Heterotrimeric G Protein Mutants (Pages 37-43)
Aequorin Luminescence-Based Functional Calcium Assay for Heterotrimeric G-Proteins in Arabidopsis (Pages 45-54)
Methods for Analysis of Disease Resistance and the Defense Response in Arabidopsis (Pages 55-66)
Fusarium oxysporum Infection Assays in Arabidopsis (Pages 67-72)
Analysis of Unfolded Protein Response in Arabidopsis (Pages 73-80)
Functional Analysis of Heterotrimeric G Proteins in Chloroplast Development in Arabidopsis (Pages 81-87)
G Protein Signaling in UV Protection: Methods for Understanding the Signals in Young Etiolated Seedlings (Pages 89-101)
Functional Analysis of Small Rab GTPases in Cytokinesis in Arabidopsis thaliana (Pages 103-112)
In Vivo Localization in Arabidopsis Protoplasts and Root Tissue (Pages 113-120)
Analysis of Protein Prenylation and S-Acylation Using Gas Chromatography–Coupled Mass Spectrometry (Pages 121-134)
In Vitro Myristoylation Assay of Arabidopsis Proteins (Pages 135-139)
Assaying Protein S-Acylation in Plants (Pages 141-146)
In Vitro Prenylation Assay of Arabidopsis Proteins (Pages 147-160)
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