Larones Medically Important Fungi A Guide to Identification 7th Edition by Lars Westblade, Eileen Burd, Shawn Lockhart, Gary Procop 1683674405 9781683674405

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ISBN 10: 1683674405

ISBN 13: 9781683674405

Author: Lars F. Westblade; Eileen M. Burd; Shawn R. Lockhart; Gary W. Procop

 

With a new team of authors, Larone’s Medically Important Fungi, Seventh Edition, continues the longstanding tradition of high-quality content to expand your knowledge and support your work in clinical mycology by:

• Providing detailed descriptions of the major mycoses as viewed in patients’ specimens by direct microscopic examination of stained slides
• Offering a logical step-by-step process for identification of cultured organisms, utilizing detailed descriptions, images, pointers on organisms’ similarities and distinctions, and selected references for further information
• Covering more than 150 of the fungi most commonly encountered in the clinical mycology laboratory, including new entries for Emergomyces, Metarhizium anisopliae, Rasamsonia argillacea, Rhinocladiella mackenziei, Schizophyllum commune, and Thermothelomyces thermophilus
• Presenting details on each organism’s pathogenicity, growth characteristics, relevant biochemical reactions, and microscopic morphology, illustrated with photomicrographs, unique and elegant drawings, and color photos of colony morphology and various test results
• Explaining changes in fungal taxonomy and nomenclature that are due to information acquired through molecular taxonomic studies of evolutionary fungal relationships
• Providing basic information on molecular diagnostic methods, e.g., nucleic acid amplification and sequencing, MALDI-TOF mass spectrometry, and other commercial platforms
• Including an extensive section of easy-to-follow lab protocols, a comprehensive list of media and stain procedures, guidance on collection and preparation of patient specimens, and an illustrated glossary

With Larone’s Medically Important Fungi: A Guide to Identification, both novices and experienced professionals in clinical microbiology laboratories can confidently identify commonly encountered fungi.

Larones Medically Important Fungi A Guide to Identification 7th Table of contents:

PART I: Direct Microscopic Examination of Clinical SpecimensDirect Microscopic Examination of Clinical Specimens

Introduction

Histological Terminology

Tissue Reactions to Fungal Infection

Stains

Guide to Interpretation of Direct Microscopic Examination

Detailed Descriptions

Actinomycosis

Mycetoma (Actinomycotic or Eumycotic)

Nocardiosis

Mucormycosis (Zygomycosis)

Aspergillosis

Miscellaneous Hyalohyphomycoses (Other than Aspergillosis)

Dermatophytosis (Tinea, Ringworm)

Tinea versicolor

Tinea nigra

Phaeohyphomycosis

Chromoblastomycosis

Sporotrichosis

Histoplasmosis

Emergomycosis

Talaromycosis (Penicilliosis)

Blastomycosis

Paracoccidioidomycosis

Lobomycosis

Candidiasis

Trichosporonosis

Cryptococcosis

Pneumocystosis

Protothecosis

Coccidioidomycosis

Rhinosporidiosis

Adiaspiromycosis

PART II: Identification of Fungi in Culture

Guide to Identification of Fungi in Culture

FILAMENTOUS BACTERIA

MONOMORPHIC YEASTS AND YEASTLIKE ORGANISMS

THERMALLY DIMORPHIC AND/OR ENDEMIC FUNGI

THERMALLY MONOMORPHIC MOULDS

Detailed Descriptions

Filamentous Bacteria

Yeasts and Yeastlike Organisms

Thermally Dimorphic and/or Endemic Fungi

Thermally Monomorphic Moulds

PART III: Basics of Molecular Methods for Fungal Identification

Introduction

Fungal Targets

Classic Molecular Identification Methods

Polymerase Chain Reaction

Non‐Sequencing‐Based Identification Methods

MALDI‐TOF Mass Spectrometry

Signal Amplification Methods

Nucleic Acid Amplification Methods

Sequencing‐Based Identification Methods

Sanger Sequencing

Massive Parallel or Next‐Generation Sequencing

Applications of DNA Sequencing

Accurate Molecular Identification

Phylogenetic Analysis

Organism Typing

Detection of Genetic Determinants of Resistance

PART IV: Laboratory Technique

Laboratory Procedures

Collection and Preparation of Specimens

Methods for Direct Microscopic Examination of Specimens

Primary Isolation

Macroscopic Examination of Cultures

Microscopic Examination of Growth

Procedure for Identification of Yeasts

Isolation of Yeast When Mixed with Bacteria

Germ Tube Test for the Presumptive Identification of Candida albicans

Rapid Enzyme Tests for the Presumptive Identification of Candida albicans

Caffeic Acid Disk Test

Olive Oil Disks for Culturing Malassezia spp.

Conversion of Thermally Dimorphic Fungi in Culture

Sporulation Inducement Method for Apophysomyces and Saksenaea

In vitro Hair Perforation Test (for Differentiation of Trichophyton mentagrophytes and Trichophyton rubrum)

Temperature Tolerance Testing

Maintenance of Stock Fungal Cultures

Controlling Mites

Staining Methods

Acid‐Fast Modified Kinyoun Stain for Nocardia spp.

Acid‐Fast Stain for Ascospores

Ascospore Stain

Calcofluor White Stain

Giemsa Stain

Gomori Methenamine Silver (GMS) Stain

Gram Stain (Hucker Modification)

Lactophenol Cotton Blue

Lactophenol Cotton Blue with Polyvinyl Alcohol (PVA) (Huber’s PVA Mounting Medium, Modified)

Rehydration of Paraffin‐Embedded Tissue (Deparaffination)

Media

Ascospore Media

Assimilation Media (for Yeasts)

Birdseed Agar (Niger Seed Agar; Staib Agar)

Brain Heart Infusion (BHI) Agar

Buffered Charcoal‐Yeast Extract (BCYE) Agar

Canavanine Glycine Bromothymol Blue (CGB) Agar

Casein Agar

CHROMagar Candida Medium

CHROMagar Candida Plus Medium

CHROMID Candida Agar

Chromogenic Candida Agar (Brilliance Candida Agar)

Cornmeal Agar

Dermatophyte Test Medium (DTM)

Esculin Agar

Fermentation Broth for Yeasts

Inhibitory Mould Agar (IMA)

Leeming‐Notman Agar (Modified)

Lysozyme Medium

Mycosel Agar

Potato Dextrose Agar and Potato Flake Agar

Rapid Assimilation of Trehalose (RAT) Broth

Sabouraud Brain Heart Infusion Agar (SABHI Agar)

Sabouraud Dextrose Agar (SDA)

Sabouraud Dextrose Agar with 15% NaCl

Sabouraud Dextrose Broth

Starch Hydrolysis Agar

Trichophyton Agars

Tyrosine Agar

Urea Agar

Water Agar

Image Appendix

Glossary

References Cited

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