Leukemia Stem Cells Methods and Protocols 1st Edition by Cesar Cobaleda, Isidro Sanchez Garcia ISBN 1071608126 978-1071608128

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ISBN 10: 1071608126
ISBN 13: 978-1071608128
Author: César Cobaleda, Isidro Sánchez-García

The detailed volume aims to provide a comprehensive hands-on manual covering all the techniques involved in the cellular and molecular identification and characterization of both normal hematopoietic and leukemic stem cells, both from human patients and from mouse models of human leukemia. The book also covers the most frequently used experimental approaches for the generation of such stem cell-based models of human leukemia. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and comprehensive, Leukemia Stem Cells: Methods and Protocols serves as an ideal guide for researchers, both expert and novice, seeking to further our knowledge of this vital avenue of cancer research.

Leukemia Stem Cells Methods and Protocols 1st Table of contents:

Analysis and Isolation of Mouse Leukemic Stem Cells
Mass Cytometry of Hematopoietic Cells
PCR Technology to Identify Minimal Residual Disease
Next-Generation Sequencing Technology to Identify Minimal Residual Disease in Lymphoid Malignancies
Genomic Inverse PCR for Screening of Preleukemic Cells in Newborns (GIPFEL Technology)
Single-Cell Transcriptomic Analysis of Hematopoietic Cells
In-Depth Mass Spectrometry-Based Single-Cell and Nanoscale Proteomics
In Vivo Clonal Analysis of Aged Hematopoietic Stem Cells: Single-Cell Transplantation
Experimental Competitive Bone Marrow Transplant Assays
Human T-ALL Xenografts
An Experimental and Computational Protocol to Study Cell Proliferation in Human Acute Myeloid Leukemia Xenografts
Leukemic Stem Cell Culture in Cytokine-Free Medium
Ex Vivo Expansion of Adult Hematopoietic Stem and Progenitor Cells with Valproic Acid
Isolation, Culture, and Manipulation of Human Cord Blood Progenitors
Lentiviral Transduction for Optimal LSC/HSC Manipulation
Characterizing the In Vivo Role of Candidate Leukemia Stem Cell Genes
Clonal Analysis of Patient-Derived Samples Using Cellular Barcodes
Arrayed Molecular Barcoding of Leukemic Stem Cells
In Vivo Generation of Leukemic Stem Cells by HSC Targeting by Transgenesis
In Situ Hematopoietic Stem Cell Imaging
A Genome Editing System for Therapeutical Targeting of Stem Cells
Method for the Generation of Induced Hematopoietic Stem Cells
Modeling Leukemia Stem Cells with Patient-Derived Induced Pluripotent Stem Cells
High-Content Imaging to Phenotype Human Primary and iPSC-Derived Cells
Bioinformatic Methods to Identify Mutational Signatures in Cancer

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