Leukemia Stem Cells Methods and Protocols Methods in Molecular Biology 2185 1st edition by Cesar Cobaleda, Isidro Sanchez Garcia 1071608096 9781071608098

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ISBN 10: 1071608096

ISBN 13: 9781071608098

Author: César Cobaleda, Isidro Sánchez-García

Leukemia Stem Cells Methods and Protocols Methods in Molecular Biology 2185 1st Table of contents:

1. Introduction and Classification of Leukemias
2. Leukemia Stem Cells: Concept and Implications
3. Leukemia Stem Cell Drug Discovery
4. Analysis and Isolation of Mouse Leukemic Stem Cells
5. Mass Cytometry of Hematopoietic Cells
6. PCR Technology to Identify Minimal Residual Disease
7. Next-Generation Sequencing Technology to Identify Minimal Residual Disease in Lymphoid Malignancies
8. Genomic Inverse PCR for Screening of Preleukemic Cells in Newborns (GIPFEL Technology)
9. Single-Cell Transcriptomic Analysis of Hematopoietic Cells
10. In-Depth Mass Spectrometry-Based Single-Cell and Nanoscale Proteomics
11. In Vivo Clonal Analysis of Aged Hematopoietic Stem Cells: Single-Cell Transplantation
12. Experimental Competitive Bone Marrow Transplant Assays
13. Human T-ALL Xenografts
14. An Experimental and Computational Protocol to Study Cell Proliferation in Human Acute Myeloid Leukemia Xenografts
15. Leukemic Stem Cell Culture in Cytokine-Free Medium
16. Ex Vivo Expansion of Adult Hematopoietic Stem and Progenitor Cells with Valproic Acid
17. Isolation, Culture, and Manipulation of Human Cord Blood Progenitors
18. Lentiviral Transduction for Optimal LSC/HSC Manipulation
19. Characterizing the In Vivo Role of Candidate Leukemia Stem Cell Genes
20. Clonal Analysis of Patient-Derived Samples Using Cellular Barcodes
21. Arrayed Molecular Barcoding of Leukemic Stem Cells
22. In Vivo Generation of Leukemic Stem Cells by HSC Targeting by Transgenesis
23. In Situ Hematopoietic Stem Cell Imaging
24. A Genome-Editing System for Therapeutical Targeting of Stem Cells
25. Method for the Generation of Induced Hematopoietic Stem Cells
26. Modeling Leukemia Stem Cells with Patient-Derived Induced Pluripotent Stem Cells
27. High Content Imaging to Phenotype Human Primary and iPSC-Derived Cells
28. Bioinformatic Methods to Identify Mutational Signatures in Cancer
    

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Cesar Cobaleda,Isidro Sanchez Garcia,Leukemia,Methods