Urinary Biomarkers 1st Edition by Samanta Salvi, Valentina Casadio 9781071613566

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ISBN 13: 9781071613566

Author: Samanta Salvi, Valentina Casadio

This volume describes important methods, prools, and techniques used for studying urinary biomarkers. Chapters detail different alterations use to studied different types of cancers and physiological conditions. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Urinary Biomarkers: Methods and Prools aims to be a useful practical guide to researches to help further their study in this field.

Urinary Biomarkers 1st Table of contents:

1. Introduction to Urinary Biomarkers in Tumors – Explores the potential of urine as a non-invasive source for cancer diagnostics.
2. Urinary Cell-free DNA Integrity Analysis – Discusses how the structural characteristics of cell-free DNA in urine can serve as indicators of tumor presence and progression.
3. Mutational Analysis in Urinary Cell-Free DNA: KRAS in Colorectal Cancer – Highlights the detection of KRAS mutations in urine for colorectal cancer screening.
4. Fluorescence In Situ Hybridization in Urine Samples (UroVysion Kit) – Examines the application of FISH in identifying chromosomal abnormalities linked to urinary tract tumors.
5. Analysis of Copy Number Variation in Urine: c-Myc Evaluation Using a Real-Time PCR Approach – Details methods for detecting oncogenic amplifications in urinary DNA.
6. Urinary microRNA and mRNA in Tumors – Discusses the role of small RNA molecules as promising urinary biomarkers for early cancer detection.
7. Long Non-Coding RNAs as Innovative Urinary Diagnostic Biomarkers – Explores the diagnostic potential of lncRNAs in cancer.
8. Urinary Nucleic Acid in Tumor: Bioinformatics Approaches – Covers computational methods to analyze urinary nucleic acids for diagnostic insights.
9. PCA3 in Prostate Cancer – Describes the diagnostic utility of PCA3, a prostate-specific RNA marker in urine.
10. Urinary Exosomes in Prostate Cancer – Investigates exosomal contents in urine as biomarkers for prostate cancer.
11. Urinary Biomarkers in Bladder Cancer – Reviews biomarkers specific to bladder cancer for diagnosis and monitoring.
12. Telomerase Activity Analysis in Urine Sediment for Bladder Cancer – Explains the significance of telomerase activity in urinary cells as a cancer biomarker.
13. Protocols for Preparation and Mass Spectrometry Analysis of Clinical Urine Samples – Guides the process for identifying candidate biomarkers, particularly in Schistosoma-associated bladder cancer.
14. Current Methods for the Isolation of Urinary Extracellular Vesicles – Overview of techniques for isolating vesicles from urine for biomarker discovery.
15. Urinary Extracellular Vesicles: Ultracentrifugation Method – Focuses on using ultracentrifugation for isolating vesicles in diagnostic applications.
16. Urinary Catecholamines as Markers in Overtraining Syndrome – Examines the role of urinary catecholamines in assessing physical stress.
17. Urinary Markers and Chronic Effect of Physical Exercise – Investigates the long-term changes in urinary markers due to exercise.
18. Urinary Metabolic Biomarkers in Cancer Patients: An Overview – Discusses metabolomic profiling of urine for cancer detection.

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Samanta Salvi,Valentina Casadio,Urinary Biomarkers